Frequently asked questions (FAQs)
How do you recommend assessing off-target effects?
Sep 19, 2017, 17:48 PM
To avoid problems with coverage using in-cell techniques, we recommend initial screening for off-target effects using cell-free methods, such as circle-seq or site-seq. Guide-seq is one unbiased live-cell method to consider. Bioinformatics-based methods for predicting off-target sites are not sufficiently comprehensive.
- Alt-R CRISPR nucleases
- Alt-R CRISPR-Cas9
- Alt-R CRISPR-Cpf1
- CRISPR genome editing