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Decrease qPCR background, improve qPCR signal

See the data yourself! Data demonstrates that probes containing a second, internal quencher provide increased signal detection and greater assay sensitivity in qPCR assays vs. single-quenched probes, such as probes with BHQ Quenchers. ZEN™ and TAO™ Double-Quenched Probes also make it more feasible to use longer probes due to the resulting lower background fluorescence.

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Choosing a qPCR assay: Inventoried or predesigned?

You may know that several companies offer inventoried qPCR assays. But did you know that these assays are often pre-manufactured and stocked, waiting perhaps for years for that order to be placed? In contrast, IDT PrimeTime® Predesigned qPCR Assays are manufactured at the time of order, and can therefore take into account sequence updates such new annotations.

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Two Quenchers Are Better Than One!

Data demonstrating that ZEN™ Double-Quenched Probes in qPCR assays provide increased signal detection and greater assay sensitivity than single-quenched probes, such as probes with BHQ Quenchers. ZEN™ Double-Quenched Probes also make it more feasible to use longer probes due to the resulting lower background fluorescence.

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IDT Competitive Advantage—Get Genes Quickly and Easily, Even from Australia

IDT provides fast, competitive gene production with great turn around delivery times to our customers as far away as Australia.

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Getting enough full-length oligo?

The coupling efficiency achieved by an oligonucleotide manufacturer has a direct effect on the quality of the oligonucleotides produced. Find out why coupling efficiency should be important to you.

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