TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Improved PCR genotyping—obtain greater precision with RNase H2 activation of assay primers

Learn about the core mechanism behind the IDT rhAmp Genotyping System and how it improves on existing 5′-nuclease PCR assay technologies.

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CRISPR-Cpf1, an alternative to Cas9 for targeting AT-rich genomes

Did you know that use of CRISPR endonuclease Cpf1 (also known as Cas12a) can greatly expand the number of target sites available for genome editing? Unlike the G-rich PAM requirement of Cas9, Cpf1 recognizes a T-rich PAM, TTTV. Not only is this enzyme useful for targeting AT-rich genomes, but it has applications in altering disease or phenotype-linked mutations in AT-rich regions through homology-directed repair. In addition, Cpf1 does not require a tracrRNA for function. Learn more about Cpf1 editing efficiency and TTTV site frequency in this article.

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Tips for resuspending and diluting your oligonucleotides

You have received your custom oligonucleotides, and now it is time to resuspend and dilute them. Here are a few tips from our scientists that will help facilitate use of the oligos in your experiments.

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ESI mass spectrometry—why we use it for oligonucleotide quality control

IDT has been, and still is, a pioneer in using mass spectrometry for quality control in oligonucleotide synthesis. Learn about why a particular method, electrospray ionization (ESI), is used ubiquitously in our manufacturing processes.

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Consider SNPs when designing PCR and qPCR assays

NGS has led to a dramatic increase in identified SNPs. SNPs can pose a problem when they underlie primer or probe sequences used in PCR/qPCR. Learn what effect they can have and how you can minimize their impact on your PCR assays.

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