TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

A new renaissance for antisense in the era of lncRNA

Noncoding RNAs such as lncRNA, are much more prevalent in humans than protein-coding RNA. Antisense oligonucleotides (ASO), previously used for knockout experiments, are being employed to study the role of noncoding RNAs in gene regulation. ASOs provide several advantages over siRNAs (and DsiRNA) for this purpose.

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Site-directed mutagenesis—improvements to established methods

Double-stranded gBlocks Gene Fragments are an ideal tool for many mutagenesis applications. This article describes some of the relevant methods and how gene fragments can save you both time and money.

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Need a Non-Standard Modification?

IDT offers 89 modifications that are not listed in our online catalog. A few of the more popular ones are described along with information on how to order them. IDT will consider any modification you have in mind. Please make such inquiries to noncat@idtdna.com.

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Interpreting Melt Curves: An Indicator, Not a Diagnosis

Examining PCR melt curve data to determine what it can/cannot tell us about resulting PCR amplicons.

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Target Enrichment Facilitates Focused Next Generation Sequencing

The rationale and benefits of enriching subsets of the genome (target enrichment by hybrid capture) prior to sequencing.

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