TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

ESI mass spectrometry—why we use it for oligonucleotide quality control

IDT has been, and still is, a pioneer in using mass spectrometry for quality control in oligonucleotide synthesis. Learn about why a particular method, electrospray ionization (ESI), is used ubiquitously in our manufacturing processes.

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Considering SNPs when designing PCR and qPCR assays

NGS has led to a dramatic increase in identified SNPs. SNPs can pose a problem when they underlie primer or probe sequences used in PCR/qPCR. Learn what effect they can have and how you can minimize their impact on your PCR assays.

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Multiplex qPCR—how to get started

Learn how multiplex qPCR can save sample, reagent cost, and time. The article provides recommendations for multiplex qPCR assay design and experimental setup.

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Use splice junctions to your advantage in qPCR

Get recommendations for avoiding PCR amplification of genomic DNA, as well as for identifying and quantifying splice variants, in this article on designing qPCR assays that span splice junctions.

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Observing subpopulations within cloned plasmids using NGS analysis

IDT is transitioning sequence verification of our Genes products from Sanger sequencing methods to NGS. Read more to find out what the benefits are when using NGS for analysis of cloned genes.

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