TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

CRISPR-Cpf1, an alternative to Cas9 for targeting AT-rich genomes

Did you know that use of CRISPR endonuclease Cpf1 (also known as Cas12a) can greatly expand the number of target sites available for genome editing? Unlike the G-rich PAM requirement of Cas9, Cpf1 recognizes a T-rich PAM, TTTV. Not only is this enzyme useful for targeting AT-rich genomes, but it has applications in altering disease or phenotype-linked mutations in AT-rich regions through homology-directed repair. In addition, Cpf1 does not require a tracrRNA for function. Learn more about Cpf1 editing efficiency and TTTV site frequency in this article.

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Tips for resuspending and diluting your oligonucleotides

You have received your custom oligonucleotides, and now it is time to resuspend and dilute them. Here are a few tips from our scientists that will help facilitate use of the oligos in your experiments.

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ESI mass spectrometry—why we use it for oligonucleotide quality control

IDT has been, and still is, a pioneer in using mass spectrometry for quality control in oligonucleotide synthesis. Learn about why a particular method, electrospray ionization (ESI), is used ubiquitously in our manufacturing processes.

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Considering SNPs when designing PCR and qPCR assays

NGS has led to a dramatic increase in identified SNPs. SNPs can pose a problem when they underlie primer or probe sequences used in PCR/qPCR. Learn what effect they can have and how you can minimize their impact on your PCR assays.

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Multiplex qPCR—how to get started

Learn how multiplex qPCR can save sample, reagent cost, and time. The article provides recommendations for multiplex qPCR assay design and experimental setup.

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