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Multiplex qPCR—how to get started

Learn how multiplex qPCR can save sample, reagent cost, and time. The article provides recommendations for multiplex qPCR assay design and experimental setup.

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Use splice junctions to your advantage in qPCR

Get recommendations for avoiding PCR amplification of genomic DNA, as well as for identifying and quantifying splice variants, in this article on designing qPCR assays that span splice junctions.

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Observing subpopulations within cloned plasmids using NGS analysis

IDT is transitioning sequence verification of our Genes products from Sanger sequencing methods to NGS. Read more to find out what the benefits are when using NGS for analysis of cloned genes.

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Planning to work with aptamers?

We are often asked whether IDT manufactures aptamers. The answer is, yes! IDT does synthesize aptamers and aptamer libraries, and there are already 100s of published research papers describing the successful use of such sequences manufactured by IDT. Learn about aptamers, SELEX, and how IDT can assist you with reagents for your aptamer applications.

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CRISPR guide RNA format affects genome editing outcomes

Learn how use of different formats for the guide RNAs associated with CRISPR-Cas9 genome editing can lead to different editing outcomes. The optimized, short RNA oligos that make up the crRNA and tracrRNA components of the Alt-R™ CRISPR-Cas9 System outperform other CRISPR guide RNA formats. Unlike DNA expression constructs, short RNA oligos also are unable to incorporate into the target genome for cleaner editing results.

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