TECHVAULT > DECODED ARCHIVE > PIPET TIPS

CRISPR-Cas9 mediated HDR: Tips for successful experimental design

Discover how type of donor template, template design, and choice of PAM site can affect efficiency of Cas9-mediated homology-directed repair (HDR). Then read the linked application note for detailed, step-wise guidance to maximize HDR rates in your own genome editing experiments.

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Protocols for CRISPR genome editing in your model system

Looking for CRISPR genome editing protocols? Read about our growing library of protocols and user methods—we may have what you need to get started. And, if you have a novel protocol for using Alt-R CRISPR RNA and/or nucleases, find out how to share it with the research community.

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Storing oligos: 7 things you should know

Lab tips: Researchers often have questions about the stability of their oligos and how best to store them. Review these important considerations and supporting data, which were generated from an ongoing, multi-year, longitudinal stability study.

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Tips for working with gBlocks® Gene Fragments

Working with IDT custom, synthetic dsDNA fragments? Get these tips from our scientists on the best ways to resuspend, quantify, and calculate copy number of gBlocks® Gene Fragments.

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Considerations when adopting published sequences for your own qPCR assay

Lab tip: Published papers that use qPCR applications are a resource of vetted assay sequences. These can be co-opted and converted to more sensitive, double-quenched probes for use in your own experiments. Before ordering, though, go through this checklist to ensure that these sequences will work well in your assays, and consider up-to-date PrimeTime Predesigned qPCR Assays with guaranteed efficiencies of >90%.

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