Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency

Looking for ways to increase the genome editing activity in your CRISPR experiments? This quick test suggests that spending a little extra time to anneal CRISPR RNAs will provide improvement.

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Could your PCR be affected by contamination?

Learn how to prevent false amplification from DNA contamination, and how to address contamination when it does occur.

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Do your qPCR assays come with sequence information? They should. Here Is why.

qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

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CRISPR genome editing: 5 considerations for target site selection

Read how your genome editing experiments can be improved with 5 quick tips for target selection and with reagents from the Alt-R™ CRISPR-Cas9 System.

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Genome editing: How stable is my CRISPR RNA:Cas9 RNP complex?

You can safely complex CRISPR RNAs with Cas9 in advance of your experiments and store these RNPs for future use. Store CRISPR RNPs at 4°C for up to 2 weeks, or at –80°C long-term. RNP complexes stored in this way provide the same high level of genome editing as freshly complexed RNPs.

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