TECHVAULT > DECODED ARCHIVE > PIPET TIPS

Successful CRISPR genome editing in hard-to-transfect cells (i.e., Jurkat cells)

Use the conditions presented here for Clone E6-1 Jurkat cells as a starting point for optimization of CRISPR reagent delivery in cell types requiring electroporation.

Read More>

Assessing formulas that calculate Tm—do nearest-neighbor parameters matter?

Should programs that predict Tm include nearest neighbor parameters? Learn which factors are critical for Tm calculation accuracy, and which online software takes them into account.

Read More>

Designing sgRNAs for CRISPR/Cas9 experiments

Learn how CRISPR crRNA and tracrRNA sequences can be combined into a single sgRNA for simplified use in CRISPR RNA-guided genome editing.

Read More>

Could your PCR be affected by contamination?

Learn how to prevent false amplification from DNA contamination.

Read More>

Troubleshooting Polyacrylamide Gel Electrophoresis (PAGE)

Our gel electrophoresis team provides an extensive troubleshooting guide for the PAGE issues they have come across during their work.

Read More>

SUBSCRIBE

Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.

DECODED ARCHIVES