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Genome editing in C. elegans using the Alt-R™ CRISPR System

Scientists in the Dernburg lab have successfully executed CRISPR genome editing in C. elegans. Read this research profile to learn about their approach to editing, homology directed repair, and progeny screening. An abbreviated method and link to a full protocol are also provided.

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Increasing ddPCR performance in low target HIV assays

ZEN™ Double-Quenched qPCR Probes work well in ddPCR, providing Dr Matthew Learn how Dr Matthew Strain’s lab used ZEN™ Double-Quenched qPCR Probes in ddPCR, to achieve additional sensitivity through lower background in experiments with low copy number samples, where individual droplets matter. The Strain lab has published open-access protocols for their HIV assays that include these updated ZEN probe designs at www.bio-protocol.org.

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Developing onsite genotyping of Antarctic penguins

This research profile highlights the increasing need for mobile qPCR testing using an example from conservation biology. Read how PrimeTime® Custom qPCR Assays have been designed to distinguish genetic variants of Adélie penguins.

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Developing a qPCR point-of-care diagnostic for Ebola

Learn how Ubiquitome, Battelle, and IDT are developing a rapid qPCR Ebola virus test for easy use in the field. The PrimeTime qPCR assay is designed to be run on Ubiquitome’s hand-held, battery powered real-time PCR device, the Freedom4.

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Dengue, Zika transmission slowed by Wolbachia bacterium

The Eliminate Dengue Program is a multinational research organization dedicated to reducing the spread of mosquito-transmitted diseases. Learn about how IDT is supporting their work, which has recently made breakthroughs for dengue and Zika virus mitigation.

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