TECHVAULT > FAQ > APPLICATION SUPPORT > ANTISENSE OLIGOS

In choosing the antisense oligo sequence is self-dimerization a concern? If so, what is considered an acceptable complementarity (bp or delta G terms)?

You will need to be concerned with self dimerization. If the oligo has a stronger affinity for itself than your target, your experiment may not work.  To test for strong self dimerization, you can analyze your sequence at with our OligoAnalyzer SciTool by pasting your sequence into the box provided and hitting the "Dimer" button. A delta G value of -9 or more negative is problematic.   

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