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Where should I place thioate bonds to increase the stability of my oligo? Does a thioate modification interfere with annealing?

Adding phosphothioate linkages will inhibit RNase and Dnase degradation, for many antisense experiments investigators have found that adding as many as 3 phosphothioate linkages at the 5' and 3' end works well. The inclusion of these linkages does not interfere with the annealing of the oligos.

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