Why do I get an error message and surcharge message when I try and order a 35 base molecular beacon with 5'-FAM and 3' Dabcyl?
The Dabcyl modification is on a modified "C" base. Our system includes that "C" for the purposes of Tm and extinction coefficient calculations. It is not figured into the pricing of your oligo, and you can disregard the message about a 10% surcharge for your beacon. The other error messages you received are more general statements given any time the system identifies an oligo which exceeds our size limits or may require additional purification. You can also disregard these messages because your oligo does fall within our size limits for beacons. You should be able to submit your order without difficulty.