How do I resuspend my oligo to obtain 100 µM concentration?

There is a quick method to calculate how to resuspend your dry oligo to a 100 µM concentration, which is the concentration IDT recommends for your stock concentration.

To generate a 100 µM concentration you need to take the number of nanomoles of oligo in your tube and multiply by a factor of ten. The resulting number is the number of microliters of nuclease-free buffer or water to add to your tube to create a 100 µM stock concentration.

For example, if you have 28.4 nanomoles, it would be: (28.4) x (10) = 284 µL of nuclease-free buffer or water to add to your tube to create a 100 µM stock concentration.

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