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Do the positive and negative controls in the TriFECTa Kit come with primers and RT-PCR materials?

The TriFECTa Kit includes:

  • 3 Dicer-Substrate RNAi duplexes, 2nmoles each- to test knockdown you will need to order primers separately.
  • 1 negative control duplex: NC1, scrambled negative
  • 1 positive control duplex: HPRT-S1 DS Positive Control
  • 1 fluorescent transfection control duplex: TYE 563TM-labeled transfection control (formerly Cy3TM DS Transfection Control)
  •  HPLC Purification
  • Duplex resuspension buffer

The kit does not include primers or PCR materials for testing after knockdown.

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