Why do my OD measurements not match the values reported by IDT?
OD values are easily affected by a number of small variables. IDT takes two separate OD measurements to ensure accuracy of the OD calculation. These values are then averaged and a final OD value is reported. Depending upon the machine used, the sample prep conditions, and the algorithm applied to determine the OD values, there will be some variation from method to method. OD values reported by IDT should be within 20% of the actual OD value for the product depending upon the preceding variables.