TechVault

Can you use gBlocks® Gene Fragments to alter restriction sites in a plasmid vector?

May 31, 2015, 04:59 AM

Because each gBlocks® Gene Fragment can be 125−2000 bp in length, the fragments can be designed to replace a segment of vector up to 2 kb in size (allowing for 20–30 bp overlapping regions between the gene fragment and plasmid insertion site) using the Gibson Assembly® Method. This would make possible the replacement of many restriction sites, as well as adding tags and other useful sequences. Learn more about gBlocks Gene Fragments.

Categories:
  • Synthetic Biology
  • gBlocks Gene Fragments
  • Cloning and Fragment Assembly
  • Cloning
Tags:
  • restriction
  • gBlocks