TechVault

How do I determine the Tm of my sequence?

Jul 24, 2013, 22:06 PM
IDT offers a free online calculator, the OligoAnalyzer tool, that will determine the Tm of your oligonucleotide sequence, for reaction conditions you specify. Access it at http://www.idtdna.com/analyzer/Applications/OligoAnalyzer.  Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.
Categories:
  • SciTools Applications
  • Oligo Characteristics
Tags:
  • Tm
  • oligoanalyzer
  • Oligoanalyzer