How do I determine the Tm of my sequence?

Jul 24, 2013, 22:06 PM
IDT offers a free online calculator, the OligoAnalyzer tool, that will determine the Tm of your oligonucleotide sequence, for reaction conditions you specify. Access it at  Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.
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  • Oligoanalyzer