How do I resuspend my oligos?

Jul 19, 2013, 21:28 PM
We find it convenient to initially make a at 100 µM  freezer stock, which should be thawed relatively infrequently to make more dilute aliquots for short term use. Dissolve the  oligo in TE (10 mM Tris pH 8.0, 0.1 mM EDTA). Use the IDT Resuspension Calulator under the "SciTools®" tab of the IDT website for help determining volume.  Alternatively, sterile dH2O can be used. DNA kept frozen in a nuclease-free environment should be stable for years.
  • resuspend
  • resuspension
  • stock