TechVault

I am performing routine PCR, cloning, and amplicon sequencing. What scale should I use for oligo synthesis?

Jul 25, 2013, 19:35 PM
A 25 nmole scale, standard desalt oligonucleotide provides enough material for several hundred PCR reactions, so this scale should be sufficient.
Categories:
  • PCR/qPCR
  • PCR/qPCR
Tags:
  • pcr
  • cloning
  • scale
  • reactions