Should I only be concerned about sodium ions when calculating Tm?

Jul 24, 2013, 16:56 PM
Any charge will play a role in the Tm value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation. The IDT OligoAnalyzer® tool will calulate Tm for you, taking into account the presence of specific ions in the reaction buffer. Find this program under the "SciTools®" tab of the IDT website.
  • Oligo Characteristics
  • SciTools Applications
  • Tm
  • oligo analyzer