What are optimum storage conditions for a custom gene?

May 2, 2014, 19:49 PM

Store custom genes dry / lyophilized at -20°C for ideal long term stability. When ready for use, the gene tube should be briefly centrifuged to ensure that the dried DNA pellet is at the bottom of the tube. Resuspend the dry DNA pellet in 20 µL TE buffer (10 mM Tris, 0.1 mM EDTA, pH 7.5–8.0; such as IDTE) or non-DEPC treated, nuclease free water to achieve an approximate stock concentration of 100 ng/μL. Incubate at room temperature for 30 minutes, and then vortex for 20 seconds. Centrifuge the tube(s) at 10,000 x g for 1 minute. Create aliquots if desired and store resuspended DNAs (short term) at -20°C.

  • Storage
  • General Oligo Use
  • store
  • storage
  • stable
  • stability
  • lyophilize
  • gene