What conditions should I use to HPLC purify my oligos?
IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for a RP-HPLC protocol is:
Hamilton PRP-1 Reverse Phase column 250x10 mm,
Buffer A = 100% ACN (acetonitrile),
Buffer B = ACN/0.1M TEAA pH 7.3.
Run at 4 mil/min over 30 mim from 95–60% A. Monitor at 260/297 nm.