What is the minimum number of bases that can be annealed?

You can anneal an oligo of any size to its target, but longer sequences lead to more stable duplexes. A minimum of 10 bases is needed for a PCR primer to bind and remain annealed to its target long enough for extension to occur. This may be a good minimum threshold to consider if you plan to anneal DNA.
  • Duplex
  • anneal