TechVault

What is the optimal length of a primer?

Jul 26, 2013, 21:33 PM
IDT recommends you aim for primers between 18–30 bases; however the most important considerations for primer design should be their Tm value and specificity. Primers should also be free of strong secondary structures and self-complementarity.  The free IDT OligoAnalyzer SciTool can be used to evaluate these features.
Categories:
  • SciTools Applications
  • Oligo Characteristics
Tags:
  • Tm
  • Oligoanalyzer
  • primer