TechVault

What is the standard desalting procedure IDT uses for newly synthesized oligos, and what additional purification options do I have?

Jul 26, 2013, 15:40 PM
IDT uses a proprietary desalting technique that removes some truncation products and small organic contaminants from the synthesized oligonucleotide preparation. Removal of n-1mers produced during synthesis requires additional PAGE or HPLC purification. PAGE is recommended for unmodified oligos >80-100 bases, while HPLC is the preferred method for oligos modified with either fluorescent dyes or attachment chemistries.
Categories:
  • Purifications
  • DNA/RNA Synthesis
Tags:
  • hplc
  • page
  • desalt
  • n-1mer