TechVault

When calculating the Tm of my oligo, should I only use the bases which are complementary to my target or should I also include any sticky overhangs on the primer?

Jul 26, 2013, 15:58 PM
To determine the relative Tm of primers with non-complementary overhangs, only the complementary region should be taken into account. You can obtain the Tm using the free, online OligoAnalyzer tool found at www.idtdna.com/scitools.
Categories:
  • SciTools Applications
Tags:
  • Tm
  • Oligoanalyzer