Why is PAGE purification not available with fluorescent dyes?

Jul 26, 2013, 19:55 PM
We do not recommend PAGE purification for dye labeled oligos due to the presence of ammonium persulfate,  a component of the PAGE gel.  Ammonium persulfate can break linkages and may damage the ring structures that comprise many fluorescent dyes.  At IDT, PAGE gels are exposed to UV light when the oligos are excised from the gel.  UV light exposure can also damage fluorescent dyes.  HPLC purification provides a pure product without damaging fluorescent modifications.
  • Purifications
  • hplc
  • page
  • Ammonium persulfate