Top Questions

Are your Dicer-substrate siRNAs (DsiRNAs) provided ready to use, and do I need to order a transfection reagent separately?

DsiRNAs, which are supplied duplexed and dried, must only be resuspended before use (see Resuspension of duplexed oligonucleotides).

We do not sell transfection agents but recommend using a transfection agent to aid in DsiRNA delivery across the cell membrane. Our scientists have had success with Lipofectamine® RNAiMax (Thermo Fisher Scientific), Oligofectamine™ (Thermo Fisher Scientific), siLentFect™ (Bio-Rad), TransIT-TKO® (Mirus Bio), and other cationic-lipid transfection products, depending on the cells used.

Tags:
  • DsiRNA
  • transfection
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