How can I use FAM™ to visualize the uptake of my oligo into a cell, and can it be added to phosphorothioate-modified bases?
Attaching a fluorescent label to monitor cellular update of oligos is very common. It does not matter whether FAM is attached 5’, 3', or internally. Generally modifications are added to the 5’ end as synthesis is easier, and therefore often cheaper. FAM can be added to phosphorothioate modified bases.