TechVault PRODUCT DNA/RNA Synthesis Gene Silencing and Small RNAs Genotyping GMP Manufacturing Modifications Molecular Biology Reagents NextGen Sequencing PCR/qPCR Purification QC Information RNase H2 SciTools Applications Synthetic Biology APPLICATION Cloning and Fragment Assembly Gene Silencing and Small RNAs General Oligo Use Genotyping Mutagenesis NextGen Sequencing Oligo Characteristics PCR/qPCR CUSTOMER SUPPORT Account Ordering Pricing and Payment Shipping and Turnaround FAQ/ Tags Can a phosphorothioated oligo for antisense studies be used in medium containing serum, and what will be its half life? Can IDT make a DNA oligo with a mixture of phosphodiester and phosphorothioate bonds? Do phosphorothioate bonds interfere with annealing? How do I order a phosphorothioate modified oligo? What types of modifications are routinely used in antisense experiments? Where should I place phosphorothioate bonds to increase stability of my oligo? Will phosphothioate (PS) modifications to the ends of PCR primers provide nuclease resistance, and thus a longer half-life for the PCR product when transfected into a cell?