TechVault PRODUCT DNA/RNA Synthesis Gene Silencing and Small RNAs Genome Editing Genotyping GMP Manufacturing Modifications Molecular Biology Reagents NextGen Sequencing PCR/qPCR Purification QC Information RNase H2 SciTools Applications Synthetic Biology APPLICATION Cloning and Fragment Assembly Gene Silencing and Small RNAs General Oligo Use Genome Editing Genotyping Mutagenesis NextGen Sequencing Oligo Characteristics PCR/qPCR CUSTOMER SUPPORT Account Ordering Pricing and Payment Shipping and Turnaround FAQ/ Tags Can a phosphorothioated oligo for antisense studies be used in medium containing serum, and what will be its half life? Can IDT make a DNA oligo with a mixture of phosphodiester and phosphorothioate bonds? Do phosphorothioate bonds interfere with annealing? How do I order a phosphorothioate modified oligo? What types of modifications are routinely used in antisense experiments? Where should I place phosphorothioate bonds to increase stability of my oligo? Will phosphothioate (PS) modifications to the ends of PCR primers provide nuclease resistance, and thus a longer half-life for the PCR product when transfected into a cell?