Gene cloning using PCR is a well-established and effective technique for constructing native DNA sequences. However, when modifying or assembling novel genes, traditional PCR methods can be very inefficient. This often leads researchers to use a costly gene synthesis service to generate such constructs. This webinar presents a novel, rapid, and reliable method to build and clone the genes you need at a fraction of the cost of full gene synthesis services, using gBlocks™ Gene Fragments (IDT) and one of several cloning methods, including use of Gibson Assembly Master Mix (NEB). Products, cloning protocols, and applications are discussed in addition to troubleshooting recommendations and further support offered by IDT.
About the Speaker:
Adam Clore is a Scientific Applications Specialist at Integrated DNA Technologies. He has a background in microbiology and chemistry and studied the molecular genetics of hyperthermophilic archaea and their viruses for his PhD in Biology. His roles at IDT include assay design and troubleshooting, new product development, research, and scientific writing.