Quantitative real-time PCR (qPCR) is today's most widely used molecular technology, and continuous improvements to chemistries, enzymes, master mixes, and instruments have led to an increasingly reliable assay that virtually guarantees results. However, the same improvements that make it easy to generate qPCR data often conceal experimental practices that have resulted in the regular publication of data that are inconsistent, inaccurate and, sometimes, simply wrong. Incomplete reporting of experimental detail further confounds assessment of qPCR data validity, calling into question scientific conclusions that serve as a basis for further basic research and diagnostic applications.
About the Speaker: Professor Stephen Bustin
Professor Stephen Bustin BA(Mod) PhD FSB obtained his PhD from Trinity College, Dublin. He is currently Professor of Allied Health and Medicine at Anglia Ruskin University, Cambridge and Chelmsford, having been Professor of Molecular Science at Queen Mary, University of London. He was appointed visiting Professor of Molecular Biology at the University of Middlesex in 2006. Prof Bustin began using PCR in 1987 and acquired his first qPCR instrument in 1997. He has published numerous peer-reviewed papers that describe and use this technology. He wrote and edited the "A-Z of Quantitative PCR" (2004), universally acknowledged as the "qPCR Bible" and more recently "The PCR Revolution" (2011). He also led the international consortium that drew up the MIQE guidelines (2009) and is in constant demand as a speaker and teacher at international qPCR meetings and workshops.