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Using gBlocks® Gene Fragments as Synthetic Templates for qPCR

Double-stranded, sequence-verified gBlocks® Gene Fragments are a new alternative to sin­gle-stranded oligonucleotides that enable creation of long, completely custom DNA se­quences. Available in lengths of 125--2000 bp, they function exactly like a double-stranded PCR product in applications, while offering all of the sequence flexibility of custom, chemically synthesized DNA. One application involves incorporating several PCR targets onto a single gBlocks Gene Fragment for use as a control template in qPCR experiments.