Blocked-Cleavable Primer and RNase H2 Publications

RNase H2–Dependent PCR (rhPCR)

  1. Boucard AA, Maxeiner S, Südhof TC. (2014) Latrophilins function as heterophilic cell-adhesion molecules by binding to teneurins: regulation by alternative splicing. J Biol Chem, 289(1):387–402.
    The scientists combined reverse transcription with RNase H2-dependent qPCR using blocked cleavable primers (described by Dobosy et al, 2011) to measure levels of alternatively spliced transcripts of latrophilins and of teneurins.
  2. Dobosy JR, Rose SD, Beltz KR, Rupp SM, Powers KM, Behlke MA, Walder JA . (2011) RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers. BMC Biotechnol, 11(80):1–18.

Other Applications of Blocked-Cleavable Primers

  1. Hicke B, Pasko C, et al. (2012) Automated detection of toxigenic Clostridium difficile in clinical samples: isothermal tcdB amplification coupled to array-based detection. J Clin Microbiol, 50(8):2681–2687.
    Use of RNase H2–dependent PCR to amplify and detect the tcdB gene in a region of the pathogenicity locus of C. difficile  with unknown sequence variation and insertions/deletions.
  2. Ao W, Aldous S, et al. (2012) Rapid detection of rpoB gene mutations conferring rifampin resistance in Mycobacterium tuberculosis. J Clin Microbiol, 50(7):2433–2440.
    Initiation of isothermal helicase-dependent amplification of a rpoB gene sequence by RNase H2–mediated cleavage of blocked DNA primers. 

​IDT authors indicated in bold type.