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RxnReady Oligos—get your oligos premixed!

Oligonucleotide mixes can be useful when performing multiplex PCR, or when generating sets of insertions or deletions through site-directed mutagenesis. Multiple probes in a single mix might also be used for FISH and similar chromosomal painting applications. Making these mixes requires bench time and can result in errors due to contamination. However, IDT can make oligonucleotide mixes for you. For a minimal mixing fee, you can have 2–6 standard desalted DNA oligonucleotides premixed in a single tube according to your specifications. RxnReady oligos may even be ordered with certain modifications (see table, below). Yields range between 5 and 50 nmoles, and your mixed oligos can be shipped dried down or hydrated (in RNase-free water* or IDTE, pH 8.0).

To order RxnReady oligo mixes, submit 2−6 sequences using the RxnReady ordering tool found under Custom DNA Oligos on our website. You can also order primer mixes through the PrimerQuest® Tool in the IDT Tools suite (www.idtdna.com/scitools). For more complex oligo mixes, including those containing more than 6 sequences, email customquotes@idtdna.com.

*To ensure longer stability of oligos, IDT recommends resuspension in IDTE, pH 8.0.

Available modifications for RxnReady sequences

Mod code Description
/5AmC12/ 5 ́ Amino Modifier C12
/5AmC6/ 5 ́ Amino Modifier C6
/5Biosg/ 5 ́ Biotin
/5deoxyI/ 5 ́ deoxyInosine
/5deoxyU/ 5 ́ deoxyUridine
/5Phos/ 5 ́ Phosphorylation
/ideoxyI/ Internal deoxyInosine
/ideoxyU/ Internal deoxyUridine

Additional reading

DECODED Online newsletter articles—Written by scientists, these articles provide tips for conducting more effective experiments, and give overviews of the latest techniques and applications used in the field. They also include in depth research profiles and citation summaries that describe research done by your colleagues who already successfully using IDT products.

Oligo handling support and educational content—View articles specifically about oligonucleotide handling, applications, protocols, and products.

See all DECODED online articles.

Author: Ellen Prediger, PhD, is a senior scientific writer at IDT.

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