Genome Editing
Support and Educational Content

Getting started with Alt-R® CRISPR-Cas9 genome editing

The Alt-R CRISPR-Cas9 system is a potent tool for genome editing applications. If you saw our webinar, New RNA tools for optimized CRISPR-Cas 9 genome editing, presented by research scientist Ashley Jacobi, you would have already seen the data showing excellent performance and reduced cell toxicity.

In our second webinar, discussing the Alt-R CRISPR-Cas9 System, Increase efficiency of genome editing with the Alt-R® CRISPR-Cas9 System: design and use, Dr Garrett Rettig showed how to get started with the Alt-R System. Dr Retting covers details including the components of the system, how to design Alt-R CRISPR crRNA oligos, and shows a complete CRISPR protocol from the user guide. If you are ready to get started with genome editing, or to improve your existing protocols, check out the recorded presentation below. You can also visit the product page to view performance data and obtain the user guide.


Product focus—genome editing with Alt-R® CRISPR Reagents

Alt-R CRISPR-Cas9 System

The Alt-R CRISPR-Cas9 System includes all the reagents needed for successful genome editing. Based on the natural S. pyogenes CRISPR-Cas9 system, the Alt-R CRISPR-Cas9 System offers numerous advantages over alternative methods:

  • Higher on-target potency than other CRISPR systems
  • Precision control with delivery of Cas9 ribonucleoprotein (RNP)
  • Efficient delivery of the RNP with lipofection or electroporation
  • No toxicity or innate immune response activation, in contrast to in vitro transcribed Cas9 mRNA and sgRNAs

Learn more about the Alt-R CRISPR-Cas9 System.


Alt-R CRISPR-Cpf1 System

The Alt-R CRISPR-Cpf1 System allows for new CRISPR target sites that are not available with the CRISPR-Cas9 System, and produces a staggered cut with a 5′ overhang. These reagents:

  • Enable genome editing in organisms with AT-rich genomes
  • Allow interrogation of additional genomic regions compared to Cas9
  • Require simply complexing the crRNA with the Cpf1 protein—no tracrRNA needed
  • Permit efficient delivery of the RNP into cells by electroporation

Learn more about the Alt-R CRISPR-Cpf1 System.


CRISPR support tools

Additional CRISPR reagents extend the ease-of-use and performance of the Alt-R system through options for fluorescent visualization, enhanced nuclease transfection, and genome editing detection.

Find out more about IDT’s entire line of CRISPR products.

Related reading

Improve your genome editing with the Alt-R® S.p. Cas9 Nuclease 3NLS and modified crRNAs—Product Spotlight: Methods for CRISPR-Cas9 genome editing are diverse, but not all of them perform equally well. Learn about the Alt-R S.p. Cas9 Nuclease 3NLS that, when used in combination the optimized Alt-R CRISPR crRNA and tracrRNA, provides a highly effective editing solution that is also easy to use.

CRISPR genome editing: 5 considerations for target site selection—Read how your genome editing experiments can be improved with 5 quick tips for target selection and with reagents from the Alt-R® CRISPR-Cas9 System.

Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency—Looking for ways to increase the genome editing activity in your CRISPR experiments? This quick test suggests that spending a little extra time to anneal CRISPR RNAs will provide improvement.

Alt-R™ CRISPR-Cas9 System ribonucleoprotein delivery optimization—Webinar: Genome editing using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) provides excellent editing efficiency, while reducing off-target editing and cell death. Watch this recorded presentation to find out how to easily generate and deliver CRISPR RNAs and Cas9 nuclease in an RNP format, using the optimized Alt-R® CRISPR-Cas9 System.


Review other DECODED Online newsletter articles on CRISPR in genome editing applications.


You can also browse our DECODED Online newsletter for additional application reviews, lab tips, and citation summaries to facilitate your research.


Author: Hans Packer, PhD, is a scientific writer at IDT.

© 2015, 2017 Integrated DNA Technologies. All rights reserved. Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners. For specific trademark and licensing information, see www.idtdna.com/trademarks.


CRISPR-Cas9 Genome Editing

With Alt-R CRISPR-Cas9 System get the core components for improved on-target editing performance, while experiencing lower toxicity, and no innate cellular response.

Increase your editing efficiency ≫


Related Content

Product page:

Alt-R CRISPR-Cas9 System

Webinar:

New RNA tools for optimized CRISPR/Cas9 genome editing

Learn how research conducted at IDT led to the development of a potent new set of CRISPR-Cas9 genome editing tools.

Poster:

Quantitative Measurement of CRISPR/Cas9 Gene Editing at the Level of Genomic DNA for sgRNA Site Selection Algorithm Development

User guide:

Alt-R CRISPR-Cas9 System User Guide