Easy resuspension and dilution of oligonucleotides

Use our free calculators to simplify your resuspension and dilution process

Save time by using these free resuspension and dilution calculators. A variety of units can be used as input values. Review the screen shots that show how these calculation tools can help you move on with your research.

Sep 21, 2012

Revised/updated Nov 16, 2016


Figure 1. Access the Resuspension and Dilution Calculators via the Tools tab on the IDT website.

The Resuspension Calculator (Figure 2) determines the volume needed to resuspend a dry-shipped oligo for a desired final concentration. You can provide the input as nmole, mg, or as OD260 with MW or extinction coefficient (provided on the spec sheet sent with the oligo). Likewise, you can specify the units of final concentration.

Note: Be sure to enter the amount shipped, not the scale ordered, as synthesis efficiency and purification will decrease the ultimate amount of oligo you receive.

WebTools Resuspension Calc Figure 2A page 11

Figure 2. The IDT Resuspension Calculator.

WebTools Dilution Calc Figure 2B page 11

Figure 3. The IDT Dilution Calculator.

The Dilution Calculator (Figure 3) provides details on how to dilute a stock solution to a desired concentration. You are given a choice of input and output concentration units (molarity or mass per volume). The Resuspension and Dilution Calculators are just two examples out of a suite of SciTools programs, provided for free use on the IDT website. Others include:

  • OligoAnalyzer® Tool—Provides primer properties like Tm and GC%, and predicts primer loops, primer dimers, and primer-primer compatibility
  • UNAFold Tool—Predicts nucleic acid folding and hybridization
  • Tools for selecting qPCR assays and DsiRNAs from a constantly updated library of designs
  • Design engines for qPCR primers and probes, RNAi, Antisense, and LNA sequences

Explore these tools, and others, in the SciTools suite here.