Molecular Beacons

Molecular Beacons are a special class of dual-labeled probes having self-complementary ends that form a stem-loop structure (hairpin) in their native state. The hairpin forces the reporter and quencher into contact (dark). Upon hybridization to target, reporter and quencher are separated and the Molecular Beacon becomes bright. Because the hairpins in these probes result in enhanced specificity for their targets, Molecular Beacons are often better able to discriminate single nucleotide polymorphisms (SNPs) than simple linear probes [1].

Molecular Beacons

Prices below include synthesis of a custom oligo (up to 45 bases in length), reporter, quencher, and HPLC purification. Turnaround time is 5–7 business days for standard Molecular Beacons.

5' Reporter DyeQuencherSynthesis ScaleMin GuaranteePrice
5' 6-FAMTM3' Dabcyl1 µmole40 nmoles$635.00 USD
250 nmole10 nmoles$440.00 USD
3' Black Hole Quencher® 11 µmole40 nmoles$760.00 USD
250 nmole10 nmoles$510.00 USD
3' Iowa Black® FQ250 nmole10 nmoles$405.00 USD
1 µmole40 nmoles$595.00 USD

5' Reporter DyeQuencherSynthesis ScaleMin GuaranteePrice
5' HEXTM 3' Iowa Black® FQ250 nmole10 nmoles$500.00 USD
1 µmole40 nmoles$660.00 USD

5' Reporter DyeQuencherSynthesis ScaleMin GuaranteePrice
5' TETTM 3' Iowa Black® FQ250 nmole10 nmoles$475.00 USD
1 µmole40 nmoles$690.00 USD

5' Reporter DyeQuencherSynthesis ScaleMin GuaranteePrice
5' TYETM5633' Iowa Black® RQ-Sp250 nmole5 nmoles$735.00 USD
1 µmole20 nmoles$1,065.00 USD

5' Reporter DyeQuencherSynthesis ScaleMin GuaranteePrice
5' TYETM6653' Iowa Black® RQ-Sp250 nmole5 nmoles$725.00 USD
1 µmole20 nmoles$1,095.00 USD

Reference

  1. Thermodynamic basis of the enhanced specificity of structured DNA probes. Bonnet et al. proc Natl Acad Sci USA 96:6171-6176 (1999).