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Top Questions

How do I determine the Tm of my sequence?

IDT offers a free online calculator, the OligoAnalyzer tool, that will determine the Tm of your oligonucleotide sequence, for reaction conditions you specify. Access it at http://www.idtdna.com/analyzer/Applications/OligoAnalyzer.  Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.
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