CRISPR systems enable targeted editing in a wide variety of organisms by introducing single- or double-strand breaks (DSBs) to DNA, which can be leveraged to introduce genomic changes. However, unintended DSBs can occur off-target at genomic loci with partial complementarity to CRISPR-Cas guide RNA (gRNA) sequences, resulting in genotype/phenotype changes in those edited cells.
Genome editing at on- and off-target sites can be quantified with multiplexed targeted next-generation sequencing (NGS) but requires bioinformatics expertise to analyze. Here, we present the rhAmpSeq CRISPR Analysis System as a streamlined end-to-end solution for designing assays, generating sequencing libraries, and analyzing NGS data for quantifying CRISPR on- and off-target editing effects without the need for bioinformatics expertise.
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