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Alternative Dyes for FISH, FRET, and qPCR

ATTO™ Dyes

ATTO™ dye labeled oligonucleotides offer additional alternatives for applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.* ATTO dyes are a group of patented fluorescent markers that feature strong absorption and sensitivity, enhanced photostability, and high fluorescence quantum yield. The ATTO dyes are perfect substitutes for dyes such as ROX™, VIC®, LIZ®, and Alexa Fluor® dyes. For qPCR applications, dye calibration will be needed for each qPCR instrument.

Catalog ATTO dyes can be attached to the 5’ or 3’ ends of 100 nmole, 250 nmole, and 1 μmole DNA and RNA oligonucleotides or duplex products. All ATTO dye labeled oligos will be HPLC purified. More information on ATTO dyes can be found at and

Order ATTO dye labeled oligonucleotides from the Custom DNA Oligos ordering page at Select the desired ATTO dye as either a 5’ or 3’ modification.

For questions about ATTO dye modifications or products not found on this list, including internal dye placement or dual-labeled probes, please contact Customer Care at

Figure 1. Strong Fluorescence of ATTO™ Dyes. Rat stomach stained with mouse anti-smooth muscle α-actin antibody followed by ATTO 488 anti-mouse IgG (green); and polyclonal rabbit anti-cytokeratin followed by ATTO 647N anti-rabbit IgG (red) [Image courtesy of ATTO-TEC GmbH].

*ATTO dyes have not been tested on Applied Biosystems genetic analyzers for use with certain fluorescent applications, such as sequencing, genotyping, and fragment analysis. Researchers must perform independent optimization for such use.