Figure 1. Access the Resuspension and Dilution Calculators via the Tools tab on the IDT website.
The Resuspension Calculator (Figure 2) determines the volume needed to resuspend a dry-shipped oligo for a desired final concentration. You can provide the input as nmole, mg, or as OD260 with MW or extinction coefficient (provided on the spec sheet sent with the oligo). Likewise, you can specify the units of final concentration.
Note: Be sure to enter the amount shipped, not the scale ordered, as synthesis efficiency and purification will decrease the ultimate amount of oligo you receive.
Figure 2. The IDT Resuspension Calculator.
Figure 3. The IDT Dilution Calculator.
The Dilution Calculator (Figure 3) provides details on how to dilute a stock solution to a desired concentration. You are given a choice of input and output concentration units (molarity or mass per volume). The Resuspension and Dilution Calculators are just two examples out of a suite of SciTools programs, provided for free use on the IDT website. Others include:
- OligoAnalyzer Tool—Provides primer properties like Tm and GC%, and predicts primer loops, primer dimers, and primer-primer compatibility
- UNAFold Tool—Predicts nucleic acid folding and hybridization
- Tools for selecting qPCR assays and DsiRNAs from a constantly updated library of designs
- Design engines for qPCR primers and probes, RNAi, Antisense, and locked nucleic acid sequences
Explore these tools, and others, in the SciTools suite here.