ATTO-labeled oligonucleotides offer additional alternatives for applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.* ATTO dyes are a group of patented fluorescent markers that feature strong absorption and sensitivity, enhanced photostability, and high fluorescence quantum yield.
The ATTO dyes are perfect substitutes for dyes such as ROX™, VIC®, LIZ®, and Alexa Fluor® dyes. For qPCR applications, dye calibration will be needed for each qPCR instrument.
Ordering ATTO dyes
Catalog ATTO dyes can be attached to 100 nmol, 250 nmol, and 1 μmol DNA and RNA oligonucleotides, or duplex products. To order, visit the DNA Oligos or RNA Oligos ordering pages, where you can choose an ATTO dye as either a 5′ or 3′ modification. All ATTO-labeled oligos from IDT will be HPLC-purified.
Catalog ATTO dye
|5' or 3' ATTO 488 (NHS Ester)||Alexa Fluor 488|
|5' or 3' ATTO 532 (NHS Ester)||VIC|
|5' or 3' ATTO 550 (NHS Ester)||NED™ and Alexa Fluor 555|
|5' or 3' ATTO 565 (NHS Ester)||PET™|
|5' or 3' ATTO Rho101 (NHS Ester)||ROX™|
|5' or 3' ATTO 590 (NHS Ester)||Alexa Fluor 594|
|5' or 3' ATTO 633 (NHS Ester)||LIZ|
|5' or 3' ATTO 647N (NHS Ester)||Alexa Fluor 647|
*ATTO dyes have not been tested on Illumina® platforms for use with certain fluorescent applications, such as sequencing, genotyping, and fragment analysis. Researchers must perform independent optimization for such use.