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Getting started with CRISPR: A review of gene knockout and homology-directed repair

CRISPR has become an increasingly popular tool for genome editing, in part because it is highly flexible and relatively easy to implement compared to other technologies. However, for scientists beginning to work with this method, the wide range of products and variety of editing approaches can be overwhelming. In this webinar, Justin Barr provides a simple explanation of the steps for planning your experiment, including guide RNA design, an overview of delivery methods, and options for measuring editing results. He also discusses how to generate specific mutations in the genome using homology-directed repair (HDR).

All Genome editing videos

IncreasinggenomeeditingefficiencyandspecificitywithoptimizedCRISPR-Cas9guideRNAs
OvercomingthechallengesofdesigningefficientandspecificCRISPRgRNAs
IncreasinggenomeeditingefficiencywithoptimizedCRISPR-Casenzymes
OptimizedmethodstouseCas9nickasesingenomeediting
HowtoorderAlt-RCRISPRcrRNAinplates
HowtoorderAlt-RCRISPRcrRNAintubes
TipsforeffectiveuseofBLASTandotherNCBItools
NewRNAtoolsforoptimizedCRISPR-Cas9genomeediting
Cpf1genomeeditingusingtheAlt-RCRISPR-Cpf1System
GettingstartedwithCRISPRAreviewofgeneknockoutandhomology-directedrepair
RibonucleoproteindeliveryofCRISPR-Cas9reagentsforincreasedgeneeditingefficiency
Increaseon-targetspecificityofCRISPRgenomeeditingusinganovelhigh-fidelityCas9

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