gBlocks Gene Fragment Libraries
gBlocks Gene Fragment Libraries are pooled gBlocks fragments that are ideal for demanding applications, such as generating recombinant antibodies or protein engineering. While they can be used directly in applications, such as in vitro transcription, or as standard or internal controls for qPCR or NGS experiments, they are most often assembled into functional genes or longer constructs by seamless assembly techniques or traditional restriction cloning. With gBlocks Gene Fragments Libraries, you can generate hundreds of thousands of constructs at a fraction of the cost that creating multiple variant libraries would require.
Ordering gBlocks libraries is just as easy as ordering standard gBlocks Gene Fragments. The tool’s interface allows N or K (G or T) mixed bases; however, they must be entered in the appropriate format. gBlocks Gene Fragments in the libraries can be 251 to 500 bp in total length, including the variable region. The variable regions can be up to 18 consecutive N or K bases long, and must be at least 125 bp from either end of the gene fragment (Figure 1).