Processing
Archer™ VARIANTPlex™ CFTR v2 panel
Comprehensive coverage for cystic fibrosis research
Interrogate all 27 exons and key intronic regions in the Cystic Fibrosis Conductance Regulator (CFTR) gene for germline variants, including detection and analysis of variants important for equitable research in diverse populations.
Detect confidently with Archer VARIANTPlex NGS Panels for DNA.
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Learn how the VARIANTPlex CFTR v2 panel can identify key genetic alterations for your research.
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Panel specifications
| Specifications | |
|---|---|
| Targeted genes | CFTR |
| Genomic alterations | SNVs, structural variants, ITDs |
| Input nucleic acid required* | ≥10 ng |
| Recommended number of reads | 100 K |
| Hands-on time | <2.5 hours |
| Total library prep time | 1.5 days |
| Platform compatibility | Illumina® |
| Reagent format | Lyophilized or liquid |
| Supported sample types | Whole blood |
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50 ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200 ng is recommended.
Gene targets
CFTR
| Gene | Regions covered |
|---|---|
| CFTR | All exons (1-27) and select intronic regions |
Interested in adding a few genes to this panel?
Customize this NGS panel by adding any of our functionally-tested designs or create a new panel that fits your exact requirements with Assay Marketplace.
Benefits
- Customize content—Use Assay Marketplace to modify any panel to fit your lab’s needs or start from scratch to keep up with the pace of discovery.
- Achieve efficiency—Parallel workflows across
all Archer panels provides streamlined genomic characterization, while single use, reaction-sized lyophilized reagents minimize hands-on time and pipetting errors.
- Detect confidently—A unique outlier detection algorithm leverages position-specific data to enable variant detection, one of the many ways that Archer Analysis empowers you to detect confidently.
- Scale easily—Liquid reagents enable automated liquid handling workflows for high volume research labs, while Anchored Multiplex PCR (AMP™) chemistry fuels reliable performance at scale.
Ready to start?
Talk with our technical sales team. Learn how the VARIANTPlex CFTR v2 panel can identify key genetic alterations for your research.
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Frequently asked questions
What is the difference between Anchored Multiplex PCR (AMP™) and traditional priming methods for DNA NGS analysis?
As opposed to traditional priming methods, AMP chemistry enables tiling primers across both strands of DNA, optimally covering targeted regions for amplification and characterization of challenging, yet relevant alterations such as internal tandem duplications. Additionally, AMP chemistry uses molecular barcode adapters that bind to DNA fragments before amplification, allowing for efficient amplification of targets even from degraded or fragmented nucleic acid input, such as DNA from FFPE tissue and ctDNA from plasma.
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