xGen™ Amplicon Panels for oncology and inherited diseases research utilize multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries in research studies (Figure 1). The PCR1+PCR2 workflow
generates NGS libraries for identifying genetic changes in the genes associated with a variety of tissues and inherited diseases (Table 1). The libraries may be quantified with conventional methods such as Qubit® (Thermo Fisher) or Agilent Bioanalyzer®
and normalized by manual pooling or normalized enzymatically with the included xGen Normalase reagents.