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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How can I check the Tm of my oligos?

The melting temperature of an oligo can be determined using our free OligoAnalyzer® Tool. Enter the sequence in the "Sequence" box and update the settings to correspond with your assay conditions.

Click "Analyze" and the melting temperature (Tm) will be displayed along with additional properties of your oligo.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.