Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How can I estimate the amount of editing obtained using the Alt-R™ CRISPR-Cas9 or -Cpf1 Systems?
We recommend using the Alt-R Genome Editing Detection Kit to determine the relative amount of editing obtained. This method uses T7 endonuclease I (T7EI) to cleave heterodimers formed in PCR amplicons following CRISPR editing.
It is important to consider that the T7EI-based assay underestimates the amount of total editing, as it does not accurately account for single-base insertions or deletions, but rather detects indels of multiple bases. Nonetheless, the Alt-R Genome Editing Detection Kit is the recommended approach for estimating editing efficiency, because of its ease of use compared to Sanger sequencing.
Categories:
CRISPR genome editing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.