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Frequently asked questions
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How does Alt-R™ S.p. HiFi Cas9 Nuclease V3 differ from the standard Alt-R S.p. Cas9 Nuclease V3?
Alt-R S.p. HiFi Cas9 Nuclease V3 was developed to reduce off-target cutting events, with similar high, on-target activity as the Alt-R S.p. Cas9 Nuclease V3.
Alt-R HiFi Cas9 nuclease can be substituted for Alt-R Cas9 nuclease in experiments that are sensitive to off-target cutting events. We recommend testing a dose-response curve using Alt-R HiFi Cas9 or other Cas9 proteins to determine the optimal dosage.
https://www.idtdna.com/pages/products/crispr-genome-editing/alt-r-crispr-enzymes