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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How does IDT calculate extinction coefficient (ɛ) and melting temperature (Tm)?

The equations we use to calculate the molar extinction coefficient and melting temperature can be found on our website at 

Use IDT free online SciTools to do the calculations for you. Try using the OligoAnalyzer Tool under SciTools.

Custom DNA & RNA

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