How many cycles of library amplification should be used with the Lotus DNA Library Prep Kit?

The number of cycles for library amplification depends on the amount of input dsDNA as well as your downstream application.

• For PCR-amplified libraries, such as those used in whole genome sequencing (WGS) applications, the minimum recommended number of PCR cycles for different dsDNA input amounts is outlined below (expected yield is ~4 nM final library):

  Input of dsDNA (ng)	Minimum PCR cycles
  ≥25	4
  10	6
  1	9

• If the constructed libraries will be subsequently used for hybridization enrichment, the minimum recommended number of PCR cycles for different dsDNA input amounts is outlined below (expected yield is ≥500 ng of material for capture):

  Input of dsDNA (ng)	Minimum PCR cycles
  250	4–5
  100	6–7
  25	8–9
  10	9–10
  1	13–14

Note that library amplification may not be required for some applications. However, if library amplification is not performed, full-length adapters must be used for adapter ligation.