Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How much reference dye should I add to the PrimeTime® Gene Expression Master Mix?

The amount of reference dye that should be added to the PrimeTime Gene Expression Master Mix depends on which real-time PCR system you are using. A table that details how much reference dye to add is provided in the protocol, PrimeTime Gene Expression Master Mix.

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qPCR & PCR