Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

FAQ categories

CRISPR genome editing 
Custom DNA & RNA 
Functional genomics 
Genes & gene fragments 
Next generation sequencing 
Optional services 
qPCR & PCR 
Reagents & kits 

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I transfected my cells with the fluorescently labeled Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550. When should I sort my cells by fluorescence activated cell sorting (FACS)?

For optimal results, we recommend sorting your cells by FACS 24 hours after delivery of the ribonucleoprotein (RNP) complex containing the Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550. The optimal signal intensity occurs at 24 hours, after which signal intensity begins to decrease.


Categories:
CRISPR genome editing

Tags:
crispr alt-r cas9