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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

I transfected my cells with the fluorescently labeled Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550. When should I sort my cells by fluorescence activated cell sorting (FACS)?

For optimal results, we recommend sorting your cells by FACS 24 hours after delivery of the ribonucleoprotein (RNP) complex containing the Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550. The optimal signal intensity occurs at 24 hours, after which signal intensity begins to decrease.